The use of <i>Artemia</i> in marine fish larviculture

Among the live diets used in the larviculture of fish and shellfish, the brine shrimp Artemia nauplii constitute the most widely used food item; i.e., over 700 metric tons of dry Artemia cysts are annually marketed worldwide for on-site hatching into 0.4 mm nauplii.Although the use of Artemia appears to be simple, considerable progress has been made in the past decade in improving and increasing its value as a larval diet for marine fish larvae. The improvements include: identification of the most appropriate strains and batches; new techniques for cyst disinfection; decapsulation and hatching; and enrichment and cold storage of nauplii. Using particulate or emulsified products rich in highly unsaturated fatty acids or n-3 HUFAs, the nutritional quality of Artemia can be further tailored to suit the predators' requirements by bio-encapsulating specific amounts of these products in the Artemia metanauplii. Application of the method of bioencapsulation, also called Artemia enrichment or boosting, has had a major impact on larviculture output, not only in terms of survival, growth and success of metamorphosis of the fish, but also with regard to their quality, e.g., reduced malformations, improved pigmentation and increased stress-resistance. Nonetheless, in many species survival rates are still marginal.For several marine fish species, the optimal dietary levels of n-3 HUFAs are still not met by enriched Artemia . Furthermore, while n-3 HUFAs might have proven most critical, it is very likely that other nutrients (e.g., other lipid classes, vitamins and free amino acids) might appear equally important and in some species even more critical. The bio-encapsulation technique can also be used for the oral delivery of hormones and therapeutics to the fish larvae. Artemia juveniles, grown to a size that suits the feeding behaviour of the growing predator and/or adult biomass collected from local saltworks or mass production units, can be used as an excellent nursery and weaning diet for most species of marine fish, eventually reducing fish mortalities, cannibalism and heterogeneous growth

Super Earth Explorer: A Coronagraphic Off-Axis Space Telescope

The Super-Earth Explorer is an Off-Axis Space Telescope (SEE-COAST) designed for high contrast imaging. Its scientific objective is to make the physico-chemical characterization of exoplanets possibly down to 2 Earth radii >. For that purpose it will analyze the spectral and polarimetric properties of the parent starlight reflected by the planets, in the wavelength range 400-1250 nmComment: Accepted in Experimental Astronom

A Very Large Number of GABAergic Neurons Are Activated in the Tuberal Hypothalamus during Paradoxical (REM) Sleep Hypersomnia

We recently discovered, using Fos immunostaining, that the tuberal and mammillary hypothalamus contain a massive population of neurons specifically activated during paradoxical sleep (PS) hypersomnia. We further showed that some of the activated neurons of the tuberal hypothalamus express the melanin concentrating hormone (MCH) neuropeptide and that icv injection of MCH induces a strong increase in PS quantity. However, the chemical nature of the majority of the neurons activated during PS had not been characterized. To determine whether these neurons are GABAergic, we combined in situ hybridization of GAD67 mRNA with immunohistochemical detection of Fos in control, PS deprived and PS hypersomniac rats. We found that 74% of the very large population of Fos-labeled neurons located in the tuberal hypothalamus after PS hypersomnia were GAD-positive. We further demonstrated combining MCH immunohistochemistry and GAD67 in situ hybridization that 85% of the MCH neurons were also GAD-positive. Finally, based on the number of Fos-ir/GAD+, Fos-ir/MCH+, and GAD+/MCH+ double-labeled neurons counted from three sets of double-staining, we uncovered that around 80% of the large number of the Fos-ir/GAD+ neurons located in the tuberal hypothalamus after PS hypersomnia do not contain MCH. Based on these and previous results, we propose that the non-MCH Fos/GABAergic neuronal population could be involved in PS induction and maintenance while the Fos/MCH/GABAergic neurons could be involved in the homeostatic regulation of PS. Further investigations will be needed to corroborate this original hypothesis

Role of the Lateral Paragigantocellular Nucleus in the Network of Paradoxical (REM) Sleep: An Electrophysiological and Anatomical Study in the Rat

The lateral paragigantocellular nucleus (LPGi) is located in the ventrolateral medulla and is known as a sympathoexcitatory area involved in the control of blood pressure. In recent experiments, we showed that the LPGi contains a large number of neurons activated during PS hypersomnia following a selective deprivation. Among these neurons, more than two-thirds are GABAergic and more than one fourth send efferent fibers to the wake-active locus coeruleus nucleus. To get more insight into the role of the LPGi in PS regulation, we combined an electrophysiological and anatomical approach in the rat, using extracellular recordings in the head-restrained model and injections of tracers followed by the immunohistochemical detection of Fos in control, PS-deprived and PS-recovery animals. With the head-restrained preparation, we showed that the LPGi contains neurons specifically active during PS (PS-On neurons), neurons inactive during PS (PS-Off neurons) and neurons indifferent to the sleep-waking cycle. After injection of CTb in the facial nucleus, the neurons of which are hyperpolarized during PS, the largest population of Fos/CTb neurons visualized in the medulla in the PS-recovery condition was observed in the LPGi. After injection of CTb in the LPGi itself and PS-recovery, the nucleus containing the highest number of Fos/CTb neurons, moreover bilaterally, was the sublaterodorsal nucleus (SLD). The SLD is known as the pontine executive PS area and triggers PS through glutamatergic neurons. We propose that, during PS, the LPGi is strongly excited by the SLD and hyperpolarizes the motoneurons of the facial nucleus in addition to local and locus coeruleus PS-Off neurons, and by this means contributes to PS genesis

Future Exoplanet Research: Science Questions and How to Address Them

Started approximately in the late 1980s, exoplanetology has up to now unveiled the main gross bulk characteristics of planets and planetary systems. In the future it will benefit from more and more large telescopes and advanced space missions. These instruments will dramatically improve their performance in terms of photometric precision, detection speed, multipixel imaging, high-resolution spectroscopy, allowing to go much deeper in the knowledge of planets. Here we outline some science questions which should go beyond these standard improvements and how to address them. Our prejudice is that one is never too speculative: experience shows that the speculative predictions initially not accepted by the community have been confirmed several years later (like spectrophotometry of transits or circumbinary planets).Comment: Invited review, accepte

Effects of feeding (omega-3) HUFA-enriched <i>Artemia</i> during a progressively increasing period on the larviculture of freshwater prawns

Larvae of the freshwater prawn (Macrobrachium rosenbergii) were reared to post-larvae in a closed recirculation system. Six treatments were designed where freshly-hatched Artemia nauplii were first given to the larvae for 3, 7, 11, 15, 17 and 25 days according to the treatment. After that (omega-3) HUFA-enriched Artemia were given until the 28th day at which time the test was terminated and evaluated. The requirement for (omega-3) Highly Unsaturated Fatty Acids (HUFA) for the larval stages of M. rosenbergii was confirmed by this experiment. Moreover, the longer the period of feeding on (omega-3) HUFA-enriched Artemia nauplii , the better the results in terms of growth, metamorphosis rate and stress resistance